Fluorescence probe and preparation method thereof, and detection method for oxytetracycline in milk



The invention discloses a fluorescence probe and a preparation method thereof, and a detection method for oxytetracycline in milk. The fluorescence probe is composed of carbon dots, which are subjected to phenol hydroxylation on surfaces and are synthesized by tannin acid as a carbon source, and Fe<3+> in an Fe<3+> solution. The detection method comprises the following steps: respectively detecting fluorescence intensities of the fluorescence probe before and after an occrycetin standard solution is added; building a linear formula according to the ratio value of the fluorescence intensities of the fluorescence probe before and after the occrycetin standard solution is added, and the concentration of oxytetracycline; detecting the fluorescence intensities of the probe before and after milk is added under the same condition; and calculating the specific content of the oxytetracycline in the milk according to the linear formula.




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Patent Citations (3)

    Publication numberPublication dateAssigneeTitle
    CN-103076316-AMay 01, 2013桂林理工大学Method for detecting trace amount of oxytetracycline by utilizing CdTe quantum dot fluorescent probe
    CN-103852460-AJune 11, 2014新疆农垦科学院基于适配体的磁纳米荧光传感器检测抗生素多残留的方法
    WO-2012129150-A2September 27, 2012University Of Maryland, Baltimore CountyPhotoluminescence augmentée par un métal à partir de nanopoints de carbone

NO-Patent Citations (2)

    LIU LIQIN等: "One-step synthesis of fluorescent hydroxyls-coated carbon dots with hydrothermal reaction and its application to optical sensing of metal ions", 《SCIENCE CHINA CHEMISTRY》, vol. 54, 31 December 2011 (2011-12-31), pages 1342 - 1347, XP019942257, DOI: doi:10.1007/s11426-011-4351-6
    严拯宇等: "生物质碳点的合成及初步用于盐酸土霉素的测定", 《无机化学学报》, vol. 30, 30 April 2014 (2014-04-30), pages 937 - 944

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