Fluorescence probe and preparation method thereof, and detection method for oxytetracycline in milk

一种荧光探针及其制备方法、牛奶中土霉素的检测方法

Abstract

The invention discloses a fluorescence probe and a preparation method thereof, and a detection method for oxytetracycline in milk. The fluorescence probe is composed of carbon dots, which are subjected to phenol hydroxylation on surfaces and are synthesized by tannin acid as a carbon source, and Fe<3+> in an Fe<3+> solution. The detection method comprises the following steps: respectively detecting fluorescence intensities of the fluorescence probe before and after an occrycetin standard solution is added; building a linear formula according to the ratio value of the fluorescence intensities of the fluorescence probe before and after the occrycetin standard solution is added, and the concentration of oxytetracycline; detecting the fluorescence intensities of the probe before and after milk is added under the same condition; and calculating the specific content of the oxytetracycline in the milk according to the linear formula.
本发明公开了一种荧光探针及其制备方法、牛奶中土霉素的检测方法,荧光探针,由以单宁酸为碳源合成的表面酚羟基化的碳点与Fe3+溶液中的Fe3+结合组成。通过分别检测加入盐酸土霉素标准溶液前后荧光探针的荧光强度,根据加入盐酸土霉素标准溶液前后荧光探针的荧光强度比值和盐酸土霉素的浓度建立线性公式,再在相同条件下检测加入牛奶前后探针的荧光强度,根据线性公式计算出牛奶中土霉素的具体含量。

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