Engineering bacterium with high L-aspartic acid alpha carboxylase activity, and its application in production of beta-alanine



The invention discloses an engineering bacterium and its application in the production of beta-alanine. A construction method of the engineering bacterium comprises the following steps: 1, inserting the coding gene of a target protein the multiple cloning site of a vector pET-30a(+) to obtain a recombinant plasmid pET30-panDB, wherein the target protein is represented by a sequence 6 in a sequence table; and 2, introducing the recombinant plasmid pET30-panDB obtained in step 1 to Escherichia coli BL21(DE3) in order to obtain the engineering bacterium. The invention also discloses a method for producing beta-alanine. The engineering bacterium is biologically converted by using L-aspartic acid as a substrate to obtain beta-alanine. The method for preparing beta-alanine has the advantages of no need of an inducer, no need of high temperature or high pressure in the whole process, mild conditions, simple operation and environmental protection, and reaches the industrial application level.




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